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Fab fragnts were prepared by proteolytic TAK-875 of trastuzumab IgG using immobilized papain in a cysteine-containing buffer as previously reported [14]. Papain is a cysteine protease derived from papaya fruit. Papain is generally considered a safe substance since it is present in papaya http://www.teamjaguarsstore.com/men-jaguars/aaron-...ic-jers ey/ , but allergies to papain-containing products applied topically have been reported [25]. Known allergy to papain or papaya fruit was included as an ineligibility criterion for the Phase I clinical trial of 111In-DTPA-trastuzumab Fab. Most importantly, papain is not released into the solution containing the Fab fragnts since it is immobilized on the agarose gel. Cysteine was removed by ultrafiltration purification and exchange of the Fab fragnts into 50 mM NaHCO3 buffer (pH 7.5). 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| Beitrag vom 06.01.2016 - 09:27 |
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| xiaohua |
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